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Image Search Results
Journal: Microbial Cell Factories
Article Title: OpenFLUX: efficient modelling software for 13 C-based metabolic flux analysis
doi: 10.1186/1475-2859-8-25
Figure Lengend Snippet: Algorithm for weighted least-square parameter estimation . OpenFLUX uses MATLAB's FMINCON to minimize the residual error ( ) between the experimental MIDs ( ) and the simulated MIDs ( ), by optimizing the free fluxes ( ), which are subjected to lower and upper boundary value constraints. is weighted by the variance matrix ( D ). The inverse compactification function is used to transform reverse flux from a numerical parameter ( v ←, [0,1] ) into a physical flux parameter ( v ← ). The null-space matrix ( Ns ) is then used to calculate the flux vector ( ) from the free fluxes ( ). Finally, is calculated using and known MIDs of the input substrates ( ). FMINCON terminates when a local minimum is found, revealing the optimum free fluxes.
Article Snippet: OpenFLUX uses
Techniques: Plasmid Preparation